Effect of allogeneic blood transfusion on neutrophil functional activity and lymphocyte cytotoxicity in recipient rabbits

The relevance of this paper is that transfusion allogeneic blood to recipient animals always associated with immunological risks. In regard, the purpose study was assess state phagocytic activity neutrophils by indicators index, number, and oxygen-dependent bactericidal activity, as well establish changes in antibody-dependent cytotoxic lymphocytes rabbits during whole transfusion. Modelling transfusions performed on five clinically healthy intravenous administration at rate 5.5 ml/kg body weight. Blood samples were taken from Days 3, 7, 23 after Neutrophil populations obtained centrifugation a double density gradient 1.077 1.093 Ficoll-Verografin. absorption phagocytes determined microscopic test. To investigate neutrophils, spontaneous test nitroblue tetrazolium performed. Antibody-dependent investigated colorimetric method. It found blood, increases simultaneous decrease their capacity. On 3 results decreased. This indicates inactivation neutrophil granulocytes first phase post-transfusion reactions. Day 23, there an increase values tetrazolium, which activation properties phagocytes. significantly decreased relative initial state, 7 it increased. An should be active synthesis antibodies late immune response. Consequently, causes response rabbits, without causing immediate long-term reactions (changes heart rate, respiratory temperature). are practical value both for scientists practising doctors who use its components acute anaemia, impaired functional coagulation factors, parasitic, oncological diseases